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二抗及鏈霉親和素

產品介紹

 
免疫熒光技術是將免疫學方法(抗原抗體特異結合)與熒光標記技術結合起來研究特異蛋白抗原在細胞內分布的方法。由于熒光素所發的熒光可在熒光顯微鏡下檢出,從而可對抗原進行細胞定位。
免疫熒光細胞化學是根據抗原抗體反應的原理,先將已知的抗原或抗體標記上熒光素制成熒光標記物,再用這種熒光抗體(或抗原)作為分子探針檢查細胞或組織內的相應抗原(或抗體)。在細胞或組織中形成的抗原抗體復合物上含有熒光素,利用熒光顯微鏡觀察標本,熒光素受激發光的照射而發出明亮的熒光,可以看見熒光所在的細胞或組織,從而對抗原或抗體進行定性、定位或定量檢測。
 

免疫熒光檢測方法根據待檢測抗原的豐度采用不同的熒光標記策略。對于高豐度的抗原,可采用熒光標記一抗直接檢測的方式;對于中等豐度的抗原,可采用熒光標記二抗的方式對待檢測的抗原進行信號放大;對于低等豐度的抗原,可采用生物素標記抗體和熒光標記鏈霉親和素的方式對待檢測的抗原進行二級信號放大。在整個免疫熒光檢測的過程中,熒光染料的性能(信號強度,穩定性,背景信號)對免疫熒光檢測的結果發揮著關鍵性的作用。早期較常用的FITC熒光素標記抗體由于其信號弱,穩定性差,目前正逐漸被新型熒光染料(如Alexa Fluor, Dylight)所取代。

Applied BioProbes公司最新推出了一系列性能優良的Andy Fluor熒光染料。這些新型熒光染料跟傳統的熒光染料(如FITC)相比,具有無可比擬的優越性。公司新推出了一系列Andy Fluor和生物素標記的二抗及相應的標記服務,為廣大科研用戶提供了廣闊的選擇。

產品特點

  • 熒光信號強;
  • 背景信號低;
  • 光穩定性好;
  • 多色熒光可供選擇;
  • 儀器兼容性好;

 

熒光標記二抗及鏈霉親和素選購指南

Secondary Antibody Streptavidin
Andy Fluor™ Dyes Other Fluorescent Labels Biotin & HRP Labels Andy Fluor™ & Cy® Dyes
Andy Fluor™ 350 Cy 3 Biotin Andy Fluor™ 350
Andy Fluor™ 405 Cy 5 HRP Andy Fluor™ 488
Andy Fluor™ 430 Cy 5.5   Andy Fluor™ 555
Andy Fluor™ 488 Cy 7   Andy Fluor™ 594
Andy Fluor™ 555 FITC   Andy Fluor™ 647
Andy Fluor™ 568     Cy®3
Andy Fluor™ 594     Cy®5
Andy Fluor™ 647      
Andy Fluor™ 680      
Andy Fluor™ 750      

Andy Fluor™ dye conjugates have brighter fluorescence than other fluorophores.

Figure 1. Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, Cy®3, and Cy®5.

Figure 2. Flow cytometry comparison of the brightness of goat anti-mouse IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, DyLight™ 488, Cy®3, DyLight™ 550, and DyLight™ 650.

Andy Fluor™ dye conjugates have better photostability than other fluorophores.


Figure 3. Comparison of the photobleaching rates of Andy Fluor™ 488 goat anti-mouse IgG (H+L) (L109B) with FITC goat anti-mouse IgG (H+L) (L146B). The cytoskeleton of HeLa cells was labeled with mouse monoclonal anti-α-tubulin antibody in combination with Andy Fluor™ 488 goat anti-mouse IgG (H+L) antibody (top series) or with mouse monoclonal anti-α-tubulin antibody in combination with FITC goat anti-mouse IgG (H+L) antibody (bottom series). The fluorescence imaging was taken at 60-second intervals (0, 60, and 120 seconds of exposure).

Figure 4. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, and then visualized with goat anti-mouse IgG antibodies conjugated with either Andy Fluor™ 488 (top, left), Andy Fluor™ 555 (top, right), Andy Fluor™ 568 (bottom, left), or Andy Fluor™ 647 (bottom, right). Nuclei are counterstained with DAPI (blue).

Figure 5. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with green-fluorescent Andy Fluor™ 488 goat anti-rabbit IgG antibody (green). Nuclei were counterstained with DAPI (blue).

Figure 6. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with either Andy Fluor™ 555 (red, left), or Andy Fluor™ 568 (red, right). Nuclei were counterstained with DAPI (blue).

Figure 7. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells and CCSP in mouse lung tissue. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, left). FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, middle and right). Nuclei were counterstained with DAPI (blue).

訂購

 

 

 

 

 

 

 

Andy Fluor™ 標記二抗

購買 貨號 產品名稱 激發/發射波長 (nm) 規格 價格(¥)

L100A
L100B
Andy Fluor™ 350 Goat Anti-Mouse IgG (H+L) Antibody 345/440 100 µg
500 µg
300
600

L101A
L101B
Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody 345/440 100 µg
500 µg
300
600

L102A
L102B
Andy Fluor™ 350 Goat Anti-Human IgG (H+L) Antibody 345/440 100 µg
500 µg
300
600

L103A
L103B
Andy Fluor™ 405M Goat Anti-Mouse IgG (H+L) Antibody 405/450 100 µg
500 µg
300
600

L104A
L104B
Andy Fluor™ 405M Goat Anti-Rabbit IgG (H+L) Antibody 405/450 100 µg
500 µg
300
600

L105A
L105B
Andy Fluor™ 405M Goat Anti-Human IgG (H+L) Antibody 405/450 100 µg
500 µg
300
600

L106A
L106B
Andy Fluor™ 430 Goat Anti-Mouse IgG (H+L) Antibody 430/540 100 µg
500 µg
300
600

L107A
L107B
Andy Fluor 430 Goat Anti-Rabbit IgG (H+L) Antibody 430/540 100 µg
500 µg
300
600

L108A
L108B
Andy Fluor™ 430 Goat Anti-Human IgG (H+L) Antibody 430/540 100 µg
500 µg
300
600

L109A
L109B
Andy Fluor™ 488 Goat Anti-Mouse IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600

L110A
L110B
Andy Fluor™ 488 Goat Anti-Rabbit IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600

L111A
L111B
Andy Fluor™ 488 Goat Anti-Human IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600

L112A
L112B
Andy Fluor™ 488 Rabbit Anti-Goat IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600

L113A
L113B
Andy Fluor™ 555 Goat Anti-Mouse IgG (H+L) Antibody 553/565 100 µg
500 µg
300
600

L114A
L114B
Andy Fluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody 553/565 100 µg
500 µg
300
600

L115A
L115B
Andy Fluor™ 555 Goat Anti-Human IgG (H+L) Antibody 553/565 100 µg
500 µg
300
600

L116A
L116B
Andy Fluor™ 568 Goat Anti-Mouse IgG (H+L) Antibody 578/605 100 µg
500 µg
300
600

L117A
L117B
Andy Fluor™ 568 Goat Anti-Rabbit IgG (H+L) Antibody 578/605 100 µg
500 µg
300
600

L118A
L118B
Andy Fluor™ 568 Goat Anti-Human IgG (H+L) Antibody 578/605 100 µg
500 µg
300
600

L119A
L119B
Andy Fluor™ 594 Goat Anti-Mouse IgG (H+L) Antibody 590/615 100 µg
500 µg
300
600

L120A
L120B
Andy Fluor™ 594 Goat Anti-Rabbit IgG (H+L) Antibody 590/615 100 µg
500 µg
300
600

L121A
L121B
Andy Fluor™ 594 Goat Anti-Human IgG (H+L) Antibody 590/615 100 µg
500 µg
300
600

L125A
L125B
Andy Fluor™ 647 Goat Anti-Mouse IgG (H+L) Antibody 650/665 100 µg
500 µg
300
600

L126A
L126B
Andy Fluor™ 647 Goat Anti-Rabbit IgG (H+L) Antibody 650/665 100 µg
500 µg
300
600

L127A
L127B
Andy Fluor™ 647 Goat Anti-Human IgG (H+L) Antibody 650/665 100 µg
500 µg
300
600

L128A
L128B
Andy Fluor™ 680 Goat Anti-Mouse IgG (H+L) Antibody: 680/700 100 µg
500 µg
300
600

L129A
L129B
Andy Fluor™ 680 Goat Anti-Rabbit IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600

L130A
L130B
Andy Fluor™ 680 Goat Anti-Human IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600

L131A
L131B
Andy Fluor™ 750 Goat Anti-Mouse IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600

L132A
L132B
Andy Fluor™ 750 Goat Anti-Rabbit IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600

L133A
L133B
Andy Fluor™ 750 Goat Anti-Human IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600

 

 

Cy 標記二抗

購買 貨號 產品名稱 激發/發射波長 (nm) 規格 價格¥

L134A
L134B
Cy® 3 Goat Anti-Mouse IgG (H+L) Antibody 550/565 100 µg
500 µg
300
600

L135A
L135B
Cy® 3 Goat Anti-Rabbit IgG (H+L) Antibody 550/565 100 µg
500 µg
300
600

L136A
L136B
Cy® 3 Goat Anti-Human IgG (H+L) Antibody 550/565 100 µg
500 µg
300
600

L137A
L137B
Cy® 5 Goat Anti-Mouse IgG (H+L) Antibody 650/667 100 µg
500 µg
300
600

L138A
L138B
Cy® 5 Goat Anti-Rabbit IgG (H+L) Antibody 650/667 100 µg
500 µg
300
600

L139A
L139B
Cy® 5 Goat Anti-Human IgG (H+L) Antibody 650/667 100 µg
500 µg
300
600

L140A
L140B
Cy® 5.5 Goat Anti-Mouse IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600

L141A
L141B
Cy® 5.5 Goat Anti-Rabbit IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600

L142A
L142B
Cy® 5.5 Goat Anti-Human IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600

L143A
L143B
Cy® 7 Goat Anti-Mouse IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600

L144A
L144B
Cy® 7 Goat Anti-Rabbit IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600

L145A
L145B
Cy® 7 Goat Anti-Human IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600

 

 

FITC 標記二抗

購買 貨號 產品名稱 激發/發射波長(nm) 規格 價格¥

L146A
L146B
FITC Goat Anti-Mouse IgG (H+L) Antibody 495/518 100 µg
500 µg
150
300

L147A
L147B
FITC Goat Anti-Rabbit IgG (H+L) Antibody 495/518 100 µg
500 µg
150
300

L148A
L148B
FITC Goat Anti-Human IgG (H+L) Antibody 495/518 100 µg
500 µg
150
300

 

 

Biotin 標記二抗

購買 貨號 產品名稱 激發/發射波長(nm) 規格 價格¥

L149A
L149B
Biotin Goat Anti-Mouse IgG (H+L) Antibody 100 µg
500 µg
150
300

L150A
L150B
Biotin Goat Anti-Rabbit IgG (H+L) Antibody 100 µg
500 µg
150
300

L151A
L151B
Biotin Goat Anti-Human IgG (H+L) Antibody 100 µg
500 µg
150
300

 

 

HRP 標記二抗

購買 貨號 產品名稱 激發/發射波長(nm) 規格 價格¥

L152A
L152B
HRP Goat Anti-Mouse IgG (H+L) Antibody 50 µg
250 µg
200
400

L153A
L153B
HRP Goat Anti-Rabbit IgG (H+L) Antibody 50 µg
250 µg
200
400

L154A
L154B
HRP Goat Anti-Human IgG (H+L) Antibody 50 µg
250 µg
200
400

 

 

鏈霉親和素耦合物

購買 貨號 產品名稱 激發/發射波長(nm) 規格 價格¥

L155A
L155B
Andy Fluor™ 350 Streptavidin 345/440 100 µg
500 µg
300
600

L156A
L156B
Andy Fluor™ 488 Streptavidin 495/520 100 µg
500 µg
300
600

L157A
L157B
Andy Fluor™ 555 Streptavidin 553/565 100 µg
500 µg
300
600

L158A
L158B
Andy Fluor™ 594 Streptavidin 590/615 100 µg
500 µg
300
600

L159A
L159B
Andy Fluor™ 647 Streptavidin 650/665 100 µg
500 µg
300
600

L160A
L160B
Cy®3 Streptavidin 555/568 100 µg
500 µg
300
600

L161A
L161B
Cy®5 Streptavidin 650/665 100 µg
500 µg
300
600